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1993

1. Adlercreutz, P.  Directing enzymatic reactions in organic media by manipulating the microenvironment of the enzyme.  In: Proceedings 6th European Congress on Biotechnology, Firenze, Italy, June 13-17, 1993. Vol II, 1993, p. TU 271

2. Adlercreutz, P.  Activation of enzymes in organic media at low water activity by polyols and saccharides.  Biochim. Biophys. Acta 1993, 1163, 144

3. Adlercreutz, P., Bloomer, S., Svensson, I., Millkvist, A., Gitlesen, T. and Mattiasson,B.  Lipase-catalyzed lipid conversions in organic media.  
In: Conference Proceedings on ?Lipases: Structure, function and protein engineering?, Elsinore, Denmark, Oct. 10-13, 1993, p.  105.

4. Adlercreutz, P. and Clapés, P.  Enzymatic peptide synthesis in organic media.  
In: Proceedings from European Symposium on Biocatalysis, Graz, Austria, Sept. 12-17, 1993, p. L-21.

5. Calvet, S., Clapés, P., Jorba, X., Xaus, N., Valencia, G. and Adlercreutz, P.  Enzymatic synthesis of X-Phe-Leu-NH2 in low water containing systems: Influence of the N-a protecting group and the reaction media composition.  Biochim Biophys Acta, 1993, 1164, 189

6. Dahlberg, L., Holst, O. and Kristjansson, J. K.  Thermostable xylanolytic enzymes from Rhodothermus marinus grown on xylan.  Appl. Microbiol. Biotechnol. 1993, 40, 63

7. Dissing, U. and Mattiasson, B.  Poly(ethyleneimine) as a phase forming polymer in aqueous two-phase systems.  Biotech. Appl. Biochem. 1993, 17, 15

8. Egger, D., Adlercreutz, P. and Kulbe, K. D.  A coupled, NAD(H)-linked enzyme system of aldose reductase and formate dehydrogenase in microemulsions.  In: Proceedings from European Symposium on Biocatalysis, Graz, Austria, Sept. 12-17, 1993, p. P-111

9. Elmroth, I., Fox, A., Holst, O., and Larsson, L.  Detection of bacterial contamination in eucaryotic production cultures using gas-chromatography-mass spectrometry.  Biotechnol. Bioeng. 1993, 42, 421

10. Galaev, I. Yu. and Mattiasson, B.  Affinity thermoprecipitation: contribution of the efficiency of ligand-protein interaction and access of the ligand.  Biotechnol. Bioeng. 1993, 41, 1101

11. Galaev, I. Y. and Mattiasson, B.  Thermoreactive water soluble polymers, nonionic surfactants, and hydrogels as reagents in biotechnology.  Enzyme Microb. Technol. 1993, 15, 354

12. Galaev, I. Yu. and Mattiasson, B.  Effect of synthetic polymers, poly (N-vinyl pyrrolidone) and poly (N-vinyl caprolactame) on elution of lactate dehydrogenase  bound to blue Sepharose.  J. Chromatogr. 648, 1993, 367

13. Ghose, S. and Mattiasson, B.  Isolation and purification of proteins  
In Thermostable enzymes. (Ed. M. N. Gupta). Narose Publ. House, New Delhi. 1993, p. 24

14. Ghose, S. and Mattiasson, B.  A two-step displacement and affinity chromatography purification in lactate dehydrogenase recovery from beef hear extract.  Biotechnol Techniques 1993, 7, 615

15. Ghose, S. and Mattiasson, B.  Protein adsorption to hydrophobic zeolite-Y - salt effects and application to protein fractionation.  Biotechnol. Appl. Biochem. 1993, 18, 311

16. Gololobov, M. Yu., Stepanov, V. M., Voyushina T. L. and Adlercreutz, P.  The second nucleophile binds to the acyl-enzyme-nucleophile complex in a chymotrypsin catalysis. Kinetic evidence,  Eur. J. Biochem. 1993, 217, 955

17. Gololobov, M. Yu., Voyushina, T. L., Stepanov, V. M. and Adlercreutz, P.  Kinetics of protease-catalyzed peptide synthesis: the effect of water content, the substrate structure and the enzyme.  In: Proceedings, 6th European Congress on Biotechnology, Firenze, Italy, June 13-17, 1993, Vol II, 1993, p TU286

18. Guoquiang, D., Kaul, R. and Mattiasson, B.  Purification of Lactobacillus bulgaricus D-lactate dehydrogenase by precipitation with an anionic polymer.  Bioseparation 3, 1993, 333

19. Gupta, M.N., Guoqiang, D. and Mattiasson, B.  Purification of endo-polygalacturonase by affinity precipitation using alginate.  Biotechnol. Appl. Biochem. 18, 1993, 321

20. Henrysson, T. and Mattiasson, B.  A microbial biosensor system for dichloromethanes.  Biodegradation 1993, 4, 101.

21. Henrysson, T. and McCarty, P.L.  Influence of the Endogeneous Storage Lipid Poly-b-hydroxybutyrate on the Reducing Power Availability During Cometabolism  
of Trichloroethylene and Naphthalene by Resting Methanotrophic Mixed Cultures.  
Appl. Environ. Microbiol. 1993, 59, 1602

22. Håkanson, H., Kyröläinen, M. and Mattiasson, B.  Portable system for continuous ex-vivo measurements of lactate  Biosensors & Bioelectronics 1993, 8, 213

23. Kaul, R. and Mattiasson, B.  Improving the shelf life of enzymes by storage under anhydrous apolar solvents.  Biotechnol. Techniques 1993, 7, 585

24. Kaul, R. and Mattiasson, B.  Storage stability of enzymes in dry apolar solvents.  
Proc. Int. Symp. on Stability and stabilzation of enzymes. Maastricht, NL. Nov 22-25, 1992. (Eds. W. J. J. van der Tweel, A.  Harder and R. M. Buitelaar). Elsevier 1993, p 361-367.

25. Kyröläinen, M., Håkanson, H. and Mattiasson, B.  Biosensor monitoring of blood lactate during open heart surgery.  Anal. Chim. Acta 1993, 279, 149

26. Larsson, L., Olsson, G., Holst, O. and Karlsson, H. T.  Oxidation of pyrite by Acidianus brierleyi: Importance of close contact between the pyrite and the microorganisms.  Biotechnol. Lett. 1993, 15, 99

27. Larsson, M. and Mattiasson, B.  Molecular weight distribution analysis of water soluble polymer using aqueous two-phasesystems.  Enzyme Engineering 11. (Eds. D. S. Clark and D. A. Estell). Ann. N.Y. Acd. Sci. vol 672. 1993, p. 649.

28. Ljunger, G., Adlercreutz, P. and Mattiasson, B.  Reactions catalyzed by PEG-modified a -chymotrypsin in organic solvents. Influence of water content and degree of  modification.  Biocatalysis 1993, 7, 279

29. Mattiasson, B., Adlercreutz, P. and Lundström, Å.  Biocatalysis in organic media at sub-zero temperatures.  In: Proceedings from ?European Symposium on Biocatalysis?, Graz, Austria, Sept. 12-17, 1993, p. L-31

30. Mattiasson, B., Adlercreutz, P. and Otamiri, M.  Polymers soluble in organic solvents as reaction aids in biocatlysis in water-poor media.  In: Proceedings from European Symposium on Biocatalysis, Graz, Austria, Sept. 12-17, 1993, p. P-27

31. Mattiasson, B. and Håkanson, H.  Sampling and sample handling - crucial steps in process monitoring and control.  Trends Biotechnol. 1993, 11, 136

32. Mattiasson, B., Linné, E. and Kaul, R.  Use of heterobifunctional ligands in affinity chromatographic processes.  In:Molecular interactions in bioseparations (Ed.T. Ngo). Plenum Press.New York, 1993, 395

33. Mattiasson, B. and Kaul, R.  Affinity precipitation  In:Molecular interactions in bioseparations. (Ed.T. Ngo ). Plenum Press, New York, 1993, pp 469

34. Mattiasson,B. and Kaul, R.  Affinity ultrafiltration for protein purification  In:Molecular interactions in bioseparations. (Ed.T. Ngo). Plenum Press, New York, 1993, p 487

35. Millkvist, A., Adlercreutz, P. and Mattiasson, B.  Enzymatic synthesis of pure 2-monoglycerides.  In: Proceedings from European Symposium on Biocatalysis, Graz, Austria, Sept. 12-17, 1993, p. P-7

36. Mushuku, T., Benhura, M. A. N., Kaul, R., Larsson, M. and Mattiasson, B.  Aqueous two-phase systems for molecular weight analyses.  Biotechnol. Techniques 1993, 7, 249

37. Ncube, I., Adlercreutz, P., Read, J. and Mattiasson, B.  Purification of rape seedling lipase and use in organic media.  Biotechnol. Appl. Biochem. 1993, 17, 327

38. Nilsson, M., Mattiasson, G. and Mattiasson, B.  Automated immunochemical bindnig assay (FLOW-ELISA) based on repeated use of an antibody column placed in a  flow-injection system.  J. Biotechnol. 1993, 31, 381

39. Olsson, G., Larsson, L., Holst, O., and Karlsson, H.T.  Desulfurization of a low sulfur coal by Acidianus brierleyi: Effects of microbial treatment on the properties of coal.  Fuel Process. Technol. 1993, 33, 83

40. Olsson, G., Larsson, L., Holst, O., and Karlsson, H.T.  Kinetics of coal desulfurization by Acidianus brierleyi.  Chem. Eng. Technol. 1993, 16, 180

41. Otamiri,M., Adlercreutz, P. and Mattiasson, B.  Ester synthesis by chymotrypsin in polymer-polymer two-phase systems in organic solvents.  In: Proceedings, 6th European Congress on Biotechnology, Firenze, Italy, June 13-17, 1993, Vol II, p TU30

42. Pinheiro,H. M., Cabral, J. M. S. and Adlercreutz, P.  Quinones as external electron acceptors in steroid 1-dehydrogenation with entrapped cells in organic medium,  Biocatalysis 1993, 7, 83

43. Shu, H.-C., Håkanson, H. and Mattiasson, B.  D-lactic acid in pork as a freshness inicator monitored by immobilized D-lactate dehydrogenase using sequential injection  analysis (SIA)  Anal. Chim. Acta. 1993, 283, 727

44. Svensson, I., Adlercreutz, P. and Mattiasson, B  Lipase catalyzed acidolysis; exchange of fatty acid in sn -1 position in phosphatidylcholine.  Conference Proceedings on ?Lipases: Structure, function and protein Engineering?. Elsinore, Denmark, Oct. 10-13, 1993, p.  97

45. Svensson, I., Adlercreutz, P., Mattiasson,B., Miesiz, Y. and Larsson, K.  Phase behaviour of aqueous systems of enzymatically modified phosphatidylcholine with one hexadecyl and one hexyl or octyl  chain.  Chem. Phys. Lipids 1993, 66, 195

46. Tocaj, A., Hof, A., Hagander, P., and Holst, O.  Fed-batch cultivation of Pseudomonas cepacia with on-line control of the toxic substrate salicylate.  Appl. Microbiol. Biotechnol. 1993, 38, 463

47. Wehtje, E., Adlercreutz, P. and Mattiasson, B.  Effects of substrate partitioning on enzyme catalysis in organic medium.  In: Proceedings from European Symposium on Biocatalysis, Graz, Austria, Sept. 12-17, 1993, p. P-19

48. Wehtje, E., Adlercreutz, P. and Mattiasson, B.  Reaction kinetics of immobilized a -chymotrypsin in organic media.  1. Influence of solvent polarity.  Biocatalysis 1993, 7, 149

49. Wehtje, E., Adlercreutz, P. and Mattiasson, B.  Reaction kinetics of immobilized a -chymotrypsin in organic media. 2. Effects of substrate partitioning.  Biocatalysis 1993, 7, 163

50. Wehtje,E., Adlercreutz, P. and Mattiasson, B.  Improved activity retention of enzymes deposited on solid supports.  Biotechnol. Bioeng. 1993, 41, 171-178

51. Wehtje,E., Svensson, I., Adlercreutz, P. and Mattiasson, B.  Continuous control of water activity during biocatalysis in organic media.  Biotechnol. Techniques 1993, 7, 873

52. Öste Triantafyllou, A., Adlercreutz, P. and Mattiasson, B. Influence of the reaction medium on enzyme activity in bioorganic synthesis. Behaviour of lipase from Candida rugosa in the  presence of polar solvents.  Biotechnol. Appl. Biochem. 1993, 17, 167

53. Öste-Triantafyllou, A., Adlercreutz, P. and Mattiasson, B. Influence of the reaction medium on enzyme activity in bioorganic synthesis.  Biotechnol. Appl. Biochem. 1993, 17, 167