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2000

Abou Hachem, M., Nordberg Karlsson, E., Bartonek-Roxå, E., Raghothama, S., Simpson, P.J., Gilbert, H.J., Williamson, M.P. Holst, O.
Carbohydrate binding modules from a thermostable Rhodothermus marinus xylanase: Cloning, expression and binding studies.
Biochem. J., 2000,345,53-60

Adlercreutz, P.
Enzymatic conversions of glycerophospholipids
In: Enzymes in Lipid Modification. U Bornscheuer (Ed.). Wiley-VCH, Weinheim, 2000, 292-306

Andersson, M, Andersson, M.M., Adlercreutz, P.
Stabilisation of choroperoxidase towards peroxide dependent inactivation.
Biocatal. Biotransform., 2000,18,457-469

Andersson, M., Breccia, J., Hatti-Kaul, R.
Stabilizing effect of chemical additives against oxidation of lactate dehydrogenase.
Biotechnol. Appl. Biochem., 2000,32,145-153

Andersson, M., Hatti-Kaul, R., Brown, W.
Dynamic and static light scattering and fluorescence studies of the interactions between lactate dehydrogenase and poly(ethyleneimine).
J. Phys. Chem. B., 2000,104,3660-3667

Arasaratnam, V., Galaev, I.Y., Mattiasson, B.
Reversibly soluble biocatalyst: optimization of trypsin coupling to Eudragit S-100 and biocatalyst activity in soluble and precipitated form.
Enzyme Microb. Technol., 2000,27,254-263

Barros, R.J., Wehtje, E., Adlercreutz, P.
Effect of mass-transfer limitations on the selectivity of immobilized alfa-chymotrypsin biocatalysts prepared for use in organic medium.
Biotechnol. Bioeng., 2000,67,319-326

Bengtsson, M., Ekström S., Drott, J., Collins, A., Csöregi, E., Marko-Varga G., Laurell, T.
Applications of Microstructured Porous Silicon as a Biocatalytic Surface.
Physica Status Solidi., 2000, 82, 533-539

Björnsson, L., Murto, M., Mattiasson, B.
Evaluation of parameters for the monitoring of an anaerobic co-digestion process.
Appl. Microbiol. Biotechnol. 2000, 54, 844-849.

Björnsson, L., Hörnsten, G., Mattiasson, B.
Evaluation of a new device for dissolved hydrogen monitoring in anaerobic digestion.
Proc. 4th International Symposium on Environmental Biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000,398-402.

Blücher, A., Nordberg Karlsson, E., Holst, O. Substrate-dependent production and some properties of a thermostable a-galactosidase from Rhodothermus marinus.
Biotechnol. Lett., 2000,22,663-669

Bontidean, I., Lloyd, J., Hobman, J., Wilson, J., Csöregi, E., Mattiasson, B.
Bacterial Metal Resistance Proteins and their Use in Biosensors for the Detection of Bioavailable Heavy Metals.
J. Inorg. Biochem, 2000, 79,225-229

Brocca, S., Persson, M., Wehtje, E., Adlercreutz, P., Alberghina, L., Lotti, M.
Mutants provide evidence of the importance of glycosidic chains in the activation of lipase 1 from Candida rugosa.
Protein Science, 2000,9,985-990

Brzozowski, Z., Pietruszka, N., Galaev, I., Mattiasson, B.
Acrylamide deivatives of starch in two-phase systems used to purify proteins. Part I. Synthesis, properties and phase diagrams.
Polymery, 2000,45,623-630 (in Polish)

Brzozowski, Z., Pietruszka, N., Galaev, I., Mattiasson, B
Acrylamide derivatives of starch in two-phase systems used to purify proteins. Part II. The use for purification of the wheat flour derived a-amylase inhibitor.
Polymery, 2000,45,707-712 (in Polish)

Christiansson, M., Stenberg, B., Holst, O.
Toxic additives - a problem upon microbial waste rubber desulphurization.
Res. Env. Biotechnol., 2000,3,11-21

Collins, A., Nandakumar, M.P., Csöregi, E., Mattiasson, B.
Monitoring of a-ketoglutarate in a fermentation process using expanded bed enzyme reactors.
In: Book of Abstracts of "Biosensors 2000", San Diego, USA, 24-26 May 2000, 123

Dainiak, M.B., Muronetz, V.I., Izumrudov, V.A., Galaev I., Mattiasson, B.
Production of monoclonal antibodies using polyelectrolyte complexes.
Analytical Biochemistry, 2000,277 58-66

Franco, T.T., Hatti-Kaul, R.
Single-step partitioning in aqueous two-phase systems.
In: Aqueous Two-Phase Systems - Methods and Protocols (R. Hatti-Kaul, ed.), Humana
Press, New Jersey, 2000,47-54

Gaspar, S., Habermuller, K., Csöregi, E., Schuhmann, W.
Biosensors based on plant peroxidases entrapped in Os-modified polypyrrole.
Sens. Actuators B., 2000,72, 63-68

Gaspar, S., Popescu, C., Gazaryan, I., Bautista, A.G., Sakharov, I., Mattiasson, B., Csöregi, E.
Biosensors based on novel plant peroxidases, a comparative study.
Electrochim. Acta, 2000, 255-264

Gaspar, G., Schuhmann, W., Csöregi, E.
Detection of hydrogen peroxide using immobilized plant peroxidases.
In: Book of Abstracts of " Biosensors 2000 The sixth World Congress on Biosensors", San Diego, USA, 24- 26 May 2000, 274

Gaspar, S., Zimmermann H., Gazarian, I., Csöreg, E., Schuhmann W.
Hydrogen peroxide sensitive biosensors based on direct electron transfer from plant peroxidase immobilised on self-assembled thiol-monolayer modified gold electrodes.
Electroanalysis, 2000,13,284-288

Guieysse, B., Bernhoft, I., Andersson, B. E., Henrysson, T., Olsson, S., Mattiasson, B.
Degradation of acenaphthene, phenanthrene and pyrene in a packed-bed biofilm reactor."
Appl. Microbiol. Biotechnol., 2000,54,826-831.

Hatti-Kaul, R. (ed. ) Aqueous Two-Phase Systems. Methods and Protocols. Humana Press, NJ., 2000

Hatti-Kaul, R.
Aqueous two-phase systems- a general overview.
In: Aqueous Two-Phase Systems - Methods and Protocols (R. Hatti-Kaul, ed.), Humana Press, New
Jersey, 2000,1-10

Hatti-Kaul, R.
Extractive bioconversion in aqueous two-phase systems.
In: Aqueous Two-Phase Systems - Methods and Protocols (R. Hatti-Kaul, ed.), Humana Press, New Jersey, 2000, 411-417

Jantsch, T.G., Mattiasson, B.
A simple spectrophotometric method for monitoring partial and total alkalinity in anaerobic processes ased on pH-indicators.
Proc. 4th International Symposium on Environmental biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000, 231-234

Kumar, A., Galaev, I.Y., Mattiasson, B.
Polymer displacement/shielding in protein chromatography.
J. Chromatogr., 2000,741,103-113

Kamihira, M., Hatti-Kaul, R., Mattiasson, B.
Integration of extraction with affinity precipitation.
In: Aqueous Two-Phase Systems - Methods and Protocols (R. Hatti-Kaul, ed.), Humana Press, New Jersey, 2000, 371-379

Li, Y.C., Larsson, E.L., Jungvid, H., Galaev, I. Y., Mattiasson, B.
Preparation and partial characterisation of neoglycoproteins by affinity chromatography.
Bioseparation. 2000,9,315-323

Lindgren, A., Ruzgas, T., Gorton, L., Csöregi, E., Ardila G., Sakharov, I., Gazaryan, I.
Biosensors based on novel peroxidases with improved properties in direct and mediated electron transfer.
Biosens. Bioelectron., 2000,15,491-497

Liu, J., Björnsson, L., Mattiasson, B.
Immobilised sludge based biosensor for BOD-measurement.
Biosensors & Bioelectronics, 2000,14,883-893

Liu, J., Mattiasson, B.
Monitoring semi-anaerobic hydrolysis of water hyacinth biomass with a BOD biosensor.
Proc. 4th International Symposium on Environmental biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000, 487-490.

Lozinsky, V., SimeneI, I., Kurskaya, E., Kulakova, V., Grinberg, V., Galaev, I., Mattiasson, B. , Khokhlov, A..
Synthesis and properties of "protein-like" copolymer
Doklady Akademii Nauk, 2000,375,637-640 (in Russian)

Lozinsky, V., SimeneI, I., Kurskaya, E., Kulakova, V., Galaev, I., Mattiasson, B, Grinberg, V., Grinberg, N., Khokhlov, A.
Synthesis of N-vinylcaprolactam polymers in aqueous media
Polymer, 2000,41,6507-6518

Mattiasson, B.
Enzyme reaction in polymer-polymer organic solvent two-phase system.
In: 'Aqueous two-phase systems. Methods and protocols. Hatti-Kaul, R. ed. Humana Press, Totowa, USA, 2000. 419-425.

Mattiasson, B., Bontidean, I., Csöregi, E., Brown, N. L., Hobman, J.L., Corbisier, P., Leth, S., Maltoni, S.
Biosensors for monitoring of heavy metal ions.
Proc. 4th International Symposium on Environmental biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000,143-146.

Mattiasson, B., Guieysse, B., Trepte, B., Pott, B.-M., Andersson, E., Welander, U., Nilsson, K., Schuur, J., Hallberg, R.
Soil remediation in cold climate - COLDREM.
Proc. 4th International Symposium on Environmental biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000,178-182

Mattiasson, B., Nandakumar, M.P.
Physicochemical basis of expanded bed adsorption for protein purification.
In ."Handbook of Bioseparation". Ed. S. Ahuja. Academic Press, 2000, 417-430.

Mawadza, C., Hatti-Kaul, R., Zvauya, R., Mattiasson, B.
Purification and characterization of cellulases produced by two Bacillus strains.
J. Biotechnology, 2000,83,177-187

Munteanu, F., Gorton, L., Lindgren, A., Ruzgas, T., Emnéus, J., Csöregi, E., Gazaryan, I., Ouporov, I., Mareeva, E., Lagrimini, L.
Direct and mediated electron transfer catalyzed by anionic tobacco peroxidase; effect of calcium ions.
Appl. Biochem. Biotech., 2000,88, 321-333

Muronetz, V.I., Kazakov, S.V., Dainiak, M B., Izumrudov, V.A., Galaev, I.Y., Mattiasson, B.
On the way of creating an artificial chaperone: Interaction of antibodies and antigens conjugated with synthetic polyanions.
Biochim. Biophys. Acta, 2000,1475,141-150

Murto, M., Mattiasson, B.
Fingerpriniting of anaerobic mixed culture by partition in two-phase system.
Proc. 4th International Symposium on Environmental biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000,411-413.

Mårtensson, O., Öste, R., Holst, O.
Lactic acid bacteria in an oat based non-dairy milk substitute - fermentation characteristics and exopolysaccharide formation.
Lebensmitt. Wiss. Tech./ Food Sci. Tech., 2000,33,525-530

Nandakumar, R., Gounot, A.M., Mattiasson, B.
Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption.
J. Biotechnology., 2000 83,211-217

Nandakumar, M.P., Nandakumar, R., Mattiasson B.
Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis.
Biotechnol. Letters., 2000, 22, 1453-1457

Nandakumar, R,. Nandakumar, M.P., Mattiasson, B.
Quantification of Nisin in Flow-Injection Immunoassay Systems.
Biosensors & Bioelectronics., 2000, 15, 241-247

Nandakumar, M.P., Pålsson, E., Gustavsson, P-E., Larsson, P.-O., Mattiasson, B.
Superporous agarose monoliths as minireactors in flow injection systems. On-line monitoring of metabolites and intracellular enzymes in microbial cultivations.
Bioseparation. 2000,9,193-202

Niculescu, M., Frébort, I., Pec, P., Galuszka P., Mattiasson B., Csöregi, E.
Amine oxidase based biosensors for histamine detection.
Electroanalysis, 2000,12,369-375

Niculescu, M., Nistor, C., Frébort I., Csöregi, E.
Electrooxidation mechanism of amine oxidase from grass pea at graphite electrodes.
Anal. Chem., 2000,72,5988-5993

Niculescu, M., Nistor, C., Ruzgas, T., Frébort, I., Pec, P., Csöregi, E.
Electrooxidation mechanism of amines at amine oxidase modified graphite electrode
In: Book of Abstracts of "Biosensors 2000", San Diego, USA, 24-26 May 2000, 268

Niculescu, M., Nistor, C., Ruzgas T., Frébort, I., Sebela, M., Pec, P., Csöregi, E
Detection of histamine and other biogenic amines using biosensors based on amine oxidase
In: Proceedings of, "European histamine research society 29th meeting", Rome, Italy, 17-21 May, 2000, 44

Niculescu, M., Nistor, C., Frébort, I., Pec, P., Mattiasson, B., Csöregi, E.
Redox hydrogel based amperometric bienzyme electrodes for fish freshness monitoring.
Anal. Chem., 2000,72,1591-1597

Nilsson, K., Mattiasson, B.
Microbial degradation of acetonitrile.
Proc. 4th International Symposium on Environmental biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000,469-472.

Persson, J., Johansson, H-O., Galaev, I., Mattiasson, B., Tjerneld, F.
Aqueous polymer two-phase systems formed by new thermoseparating polymers
Bioseparation 2000,9,105-116

Persson, M., Svensson, I., Adlercreutz, P.
Enzymatic fatty acid exchange in digalactosyldiacylglycerol,
Chem. Phys. Lipids, 2000,104, 13-2

Persson, M., Wehtje, E., Adlercreutz, P.
Immobilisation of lipases by adsorption and deposition: high protein loading gives lower water activity optimum.
Biotechnol. Lett., 2000,22,1571-1575

Pietruszka, N., Galaev, I. Kumar, A., Brzozowski, Z., Mattiasson, B.
New polymers forming aqueous two-phase polymer systems.
Biotechnology Progress 2000,16,408-415.

Pott, B-M., Berg, U., Henrysson, T., Mattiasson, B.
Bioremediation in lab-scale of creosote contaminated soil from a wood preservation site in Sweden
Res. Environ. Biotechnol. 2000,3,73-85

Pålsson, E., Nandakumar, M.P., Mattiasson, B., Larsson, P.-O.
Miniaturised expanded-bed column with low dispersion suitable for fast-flow-ELISA analyses.
Biotechnol. Letters, 2000,22,245-250.


Ramanavicius, A., Habermuller, K., Niculescu M., Vilkanauskyte, A., LapenaiteI., Mieliauskiene, R., Csöregi, E., Schuhmann, W., Laurinavicius, V.
Evaluation of glucose, glycerol and alcohol biosensors based on corresponding quinoproteins
In: Book of abstracts of "Biosensors 2000", San Diego, USA, 24-26 May 2000,3178

Ramanavicius, A., Schuhmann W., Habermüller, K., Razumiene J., Meskys, R., Marcienkeviciene, L., Bachmatova, I., Csöregi, E., Laurinavicius, V.
An oxygen independent ethanol sensor based on a polypyrrole integrated quinohemoprotein alcohol dehydrogenase.
Progr. in Coll. Polym. Sci.,2000,116,143-148

Ryabova, E., Csöregi, E., Ryabov, A.
Enzymatic chemistry of Ruthenium. Influence of biologically relevant lignads on oxidation of reduced glucose oxidase by electrochemically generated [RuIII(bpy)2XY] complexes.
J. Molecular Catalysis (Enzymatic).1, 2000,112, 139-145

Sakharov, I, Castillo, J., Areza, J. C., Galaev, I.
Purification and stability of peroxidase from leaves of African oil palm Elaies guineensis
Bioseparation, 2000,9,125-132

Samra, B. K., Galaev, I., Mattiasson, B.
Thermosensitive, reversibly cross-linking gels with a shape 'memory'
Angewandte Chemie International Edition, 2000,112,2454-2457

Schuur, J., Danielsson, A., Håkansson, T., Warfvinge, P., Mattiasson, B.
Effects of surfactants and vegetabile oil on the solubility of PAHs.
Proc. 4th International Symposium on Environmental biotechnology. 10-12 April 2000, Noordwijkerhout, The Netherlands, 2000,382.

Simkus, R., Meskys, R., Corbisier, P., Mattiasson, B., Csöregi, E.
Millihertz waveband oscillations in an unstirred bacterial culture,
Progr. Colloid. Polym. Sci. 2000,116,149-153

Skirnisdottir, S., Hreggvidsson, G. O., Hjörleifsdottir, S., Marteinsson, V. Th., Petursdottir, S.K., Holst, O., Kristjanson, J. K.
Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats.
Appl. Environ. Microbiol., 2000,66,2835-2841

Straathof, A.J.J., Adlercreutz, P.
Biocatalysis in non-conventional media.
In: Applied Biocatalysis. A J J Straathof and P Adlercreutz (Eds.)., second edition. Harwood Academic Publishers, 2000,295-316

Straathof, A. J. J., Adlercreutz, P., (eds.)
Applied Biocatalysis, second edition. Harwood Academic Publishers, 2000

Vaz, S., Persson, M., Svensson, I., Adlercreutz, P.
Lipase-catalysed fatty acid exchange in digalactosyldiacylglycerol. Improvement of yield due to the addition of phenylboronic acid.
Biocatal. Biotransform., 2000,18,1-12

Virto, C., Adlercreutz, P.
Two-enzyme system for the synthesis of 1-lauroyl-rac-glycerophosphate (lysophosphatidic acid) and 1-lauroyl-dihydroxyacetonephosphate.
Chem. Phys. Lipids, 2000,104,175-184

Virto, C., Adlercreutz, P.
Lysophosphatidylcholine synthesis with Candida antarctica lipase B (Novozyme 435).
Enz. Microb. Technol., 2000,26,630-635

Virto, C., Svensson, I., Adlercreutz, P.
Hydrolytic and and transphosphatidylation activities of phospholipase D from Savoy cabbage towards lysophosphatidylcholine.
Chem. Phys. Lipids, 2000,106,41-51

Virto, C., Svensson, I., Adlercreutz, P.
Candida antarctica lipase B-catalysed synthesis of dihydroxyacetone fatty acid esters.
Biocatal. Biotransform., 2000,18,13-29