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Assignment

Assignment

Assignment  Bioanalytical Chemistry KBTN01 - Use of biomolecules for analysis


All students will perform a group assignment (6 pers/group) concerning the use of biomolecules in analytical flow systems:


Part 1: The group will design a biosensing assay with a recognition element that either is based on the interaction of the analyte with an enzyme, antibody, DNA/RNA or cells/tissue. You must consider that the analytical solution presented in part one should preferably be implemented in a flow-Injection analysis (FIA) system later on. A sub report (2-4 pages) should be handed and emailed to to the teacher approx. Two weeks after initiation of the assignment.

Part 2: The sensing system created in part one should preferably be implemented in a FIA system for continuous analysis (other situations can occur). Design a suitable system for your analytical application and describe its constituents. Also, take into account possible pre-treatment procedures and other unit operations that might be needed. A final report of 8-10 pages shall be handed in and emailed to the teacher, containing the final solution to part 1 and 2.


Part 3: The assignment will be presented in front of the other course participants. The groups will get 10 minutes to present their work. All group members shall be prepared to present but a draw will decide who will get the opportunity to actually present the assignment work (1 pers./group).

 

The assignment in a nut shell:

1) Decide on a suitable bioanalytical method/platform

2) Chose a suitable FIA-setup (or other transportation system)

3) Implement your bioassay in the flow system, if possible.

4) Perform a theoretical optimization
     -Point out and describe your strategy of optimization

5) Describe your analytical layout once the system is optimized and ”up-and-running”

General FIA information:

Injection of sample into continuous carrier stream
Transportation of sample zone to detector flow cell
Mixing of sample and reagent (if needed)
Continuous detection of the analyte by some suitable detection technique (absorbance, electrode potential etc.)

Detection limit will improve and dispersion will decrease with:
-Larger injected sample volume (to a certain extent, detector limitations)
-Narrower tube diameter
-Choice of proper reactor geometry like knitted coil (when mixing is needed)
-Shorter tube length
-Higher flow rate (also detector limitations)
-Stopped flow (if needed/possible)