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Use in supercritical extraction methodologies

In this research program (coordinated by Dr C Turner, Uppsala university), our task at Dept Biotechnology is to select/develop thermostable glucosidases (from different sequence families and specificity groups) for use in pressurized hot water extraction or supercritical extraction methodologies. 
Promising enzymes, selected by initial trials, will be further developed by site-directed and random mutation techniques, to allow selectivity for specific bond-types coupled to high stability. 
One starting material is onion waste, and two thermostable glucosidases originating from Thermotoga neapolitana has been a good catalysts in deglycosylation of quercetin-glycosides from the onion waste to quercetin. They will be developed towards increased efficiency (or cleavage of specific bond types), both for quercetin-glycosides as well as for other interesting polyphenolic glycosides found in agricultural byproducts investigated in this project. Additional thermostable enzymes (acting on pentoses and hexoses, and stable up to 85 - 100 °C) will be tried in selected target reactions.

Contact:     Eva Nordberg Karlsson

Collaboration:    Charlotta Turner, UU
                         Per Sjöberg, UU
                         Pål Börjesson, LU
                         Maria Vredin-Johansson, UU